Version 2.77

Term Description

The average number of Chromosome 17 fluorescent signals in nuclei are determined in order to calculate the average ratio of HER2 to Chromosome 17 copy number [LOINC: 49683-6]. This term is based on, but not limited in use to, the PathVysion HER-2 DNA Probe Kit, which detects amplification of the ERBB2 gene (HER2 locus) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded human breast and gastric cancer tissue specimens. The kit contains two probes: one specific to the HER-2/neu gene locus (ERBB2 gene, 17q11.2-q12) and another targeting the alpha satellite DNA sequence at the centromeric region of chromosome 17 (17p11.1-q11.1), called CEP17 (Chromosome Enumeration Probe 17). The average number of HER2 signals [LOINC: 74860-8] along with CEP17 signals are determined by microscopic examination within the same set of nuclei. The specimen is positive for ERBB2 gene applification if the mean ratio of HER2 to CEP17 signals per nucleus is greater than or equal to 2.00.
Source: Regenstrief LOINC

Part Descriptions

LP182348-5   Chromosome 17 copy number
The Chromosome 17 DNA probe marks the centromeric region of chromosome 17 and is used as an internal control and to account for aneusomy (varying number) of chromosome 17. Source: Regenstrief LOINC

LP62864-1   FISH
FISH (fluorescence in situ hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes. FISH is often used for finding specific features in DNA for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia, FISH

Fully-Specified Name

Component
Chromosome 17 copy number/nucleus
Property
EntNum
Time
Pt
System
Tiss
Scale
Qn
Method
FISH

Additional Names

Short Name
Chr 17 copy num/nucleus EntNum Tiss FISH
Display Name
Chromosome 17 copy number/nucleus FISH (Tiss) [Entitic number]
Consumer Name Alpha Get Info
Chromosome 17 copy number/nucleus, Tissue

Basic Attributes

Class
MOLPATH
Type
Laboratory
First Released
Version 2.48
Last Updated
Version 2.73
Change Reason
Updated Component from "CEP17 signals" to "Chromosome 17 copy number" to align with current nomenclature and similar terms
Order vs. Observation
Observation
Common Test Rank Get Info
11691

Member of these Panels

LOINC Long Common Name
85904-1 Cancer pathology panel - Breast cancer specimen by CAP cancer protocols
74885-5 ERBB2 gene (HER2) duplication associated observations panel - Tissue by FISH

Language Variants Get Info

Tag Language Translation
es-ES Spanish (Spain) Señales de CEP17/núcleo:Número entítico:Punto temporal:Tejido:Qn:Hibridación in situ fluoresente (FISH)
Synonyms: Cuantitativo
es-MX Spanish (Mexico) Número de copias / núcleo del cromosoma 17:Número entitic:Punto temporal:Tejido y frotis:Cuantitativo:Hibridación fluorescente in situ (FISH)
fr-FR French (France) Chromosome 17 nombre de copie/noyaux:Nombre/entité:Ponctuel:Tissu:Numérique:FISH
it-IT Italian (Italy) CEP17, segnali/nucleo:EntNum:Pt:Tessuto:Qn:FISH
Synonyms: Ibridazione in situ fluorescente (FISH) Numero entitico Patologia molecolare Punto nel tempo (episodio) Tessuto & Strisci
nl-NL Dutch (Netherlands) aantal centromeren van chromosoom 17/kern:aantal/eenheid:moment:weefsel en uitstrijkjes:kwantitatief:FISH
Synonyms: CEP17 nucleus
pl-PL Polish (Poland) Liczba kopii chromosomu 17/jądro:liczba zawarta w:punkt w czasie:tkanka i rozmazy:ilościowy:fluorescencyjna hybrydyzacja in situ (FISH)
pt-BR Portuguese (Brazil) CEP17 sinais/núcleo::Pt:Tecido:Qn:FISH
tr-TR Turkish (Turkey) CEP17 sinyalleri/çekirdek:EntSay:Zmlı:Doku:Kant:FISH
zh-CN Chinese (China) 染色体 17 拷贝数/核:实体数量:时间点:组织与涂片:定量型:FISH
Synonyms: Fluorescent in situ hybridization;荧光原位杂交 个数;数目 分子病理学;分子病理学试验 可用数量表示的;定量性;数值型;数量型;连续数值型标尺 实体计数 时刻;随机;随意;瞬间 未作说明的组织;组织;组织 & 涂片 染色体 17 (17号染色体)拷贝(副本)数(数量、计数);17号染色体拷贝(副本)数(数量、计数);CEP17;CEP17 信号 染色体二体型+染色体三体型 细胞核

Example Units

Unit Source
{#} Example UCUM Units

LOINC Terminology Service (API) using HL7® FHIR® Get Info

CodeSystem lookup
https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=74861-6