Version 2.73

82424-3MDA5 Ab [Presence] in Serum by Line blotActive

Term Description

This term was created for, but is not limited in use to, the submitter's test strip, a membrane strip pre-coated with a specific set of antigens in parallel lines. If antibodies to any of those antigens are present in the patient's serum, they will bind to the specific antigen. The bound patient antibodies are then detected using labelled anti-human antibodies. Various manufacturers classify this method as an immunoblot technique. LOINC uses the immunoblot (IB) method to mean that some electrophoretic/chromatographic separation occurs such that position (size) of the band is part of the assessment (as in Western blot). Because this assay does not involve a separation step but rather uses strips with pre-fixed antigens, the LOINC Method is Line blot.
Source: Regenstrief LOINC

Part Description

LP220403-2   MDA5
MDA5, more formally known as interferon-induced helicase C domain-containing protein 1, is an immune receptor for viral nucleic acids and plays a major role in activating antiviral responses, including the induction of type I interferons and proinflammatory cytokines. Anti-MDA5 autoantibodies, also known as anti-CADM-140, are associated with autoimmune disorders including dermatomyositis and interstitial lung disease. [UniProt:Q9BYX4] Anti-MDA5 autoantibodies are also found in a subset of patients with juvenile dermatomyositis (JDM) who have polyarthritis and ulcerative skin disease but milder muscle disease. Anti-MDA5 assays may be useful during the work-up of patients with inflammatory disorders and may help guide treatment. [PMID:24989778] Source: Regenstrief LOINC, PMID: 24989778

Fully-Specified Name

Line blot

Additional Names

Short Name
MDA5 Ab Ser Ql Line blot
Display Name
MDA5 Ab Line blot Ql (S)
Consumer Name Alpha
MDA5 antibody, Blood

Example Answer List LL360-9

Source: Regenstrief Institute

Answer Code Score Answer ID
Positive Copyright http://snomed.info/sct ID:10828004 Positive (qualifier value) LA6576-8
Negative Copyright http://snomed.info/sct ID:260385009 Negative (qualifier value) LA6577-6

Basic Attributes

First Released
Version 2.58
Last Updated
Version 2.73
Change Reason
This term was created for an assay that uses a membrane strip pre-coated with a specific set of antigens in parallel lines. Because the antigens are seperated by electrophoresis/chromatographic separation during manufacturing, not during the test procedure itself, this test was assigned a LOINC Method of Immunoassay (IA) and not Immunoblot (IB). However, upon further review of IA, IB, and Immunoblot strip methodologies and regulatory distinctions between these, we changed the Method from IA to Line blot so that users can distinguish between these three Methods. The meaning of the term has not changed since it was created for an IB strip assay as described in the term description.
Order vs. Observation
Common US Lab Results Rank

Language Variants Get Info

zh-CNChinese (China)
MDA5 抗体:存在情况或阈值:时间点:血清:序数型:线点印迹法
nl-NLDutch (Netherlands)
MDA5 As:aanwezigheid:moment:serum:ordinaal:lijnblot
fr-FRFrench (France)
MDA5 anticorps:Présence/Seuil:Ponctuel:Sérum:Qualitatif:Blot en ligne
it-ITItalian (Italy)
MDA5 Ab:PrThr:Pt:Siero:Ord:Line blot
es-MXSpanish (Mexico)
MDA5 Ab:Presencia o umbral:Punto temporal:Suero:Ordinal:Mancha de línea
es-ESSpanish (Spain)
MDA5 Anticuerpos:PrThr:Punto temporal:Suero:Ord:Line blot

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