74676-8

Carbapenemase [Type] in Isolate by Carba NP

Active

Term Description

The Carba NP (Carbapenemase Nordmann-Poirel) test rapidly (<2 hrs) detects carbapenemase production in isolates of Enterobacteriaceae and Pseudomonas aeruginosa. Carbapenemases are detected and can be differentiated by the CarbaNP test when the test isolate produces the enzyme (in the absence of a chemical inhibitor) that hydrolyzes imipenem (a carbapenem antibiotic) into the carboxylic form of the antibiotic, thereby lowering the pH of the reaction solution which contains phenol red indicator. The acidification changes the red color of the reaction solution to orange-yellow color (positive result) that can be visualized easily with the naked eye. Carba NP testing can detect the following carbapenemase types: Klebsiella pneumoniae carbapenemase (KPC; Ambler class A); Verona integron-encoded metallo-β-lactamase (VIM), imipenemase (IMP), New Delhi metallo-β-lactamase (NDM) (all Ambler class B); and oxacillinase-48 (OXA-48; Ambler class D).

For this test, each test isolate is inoculated into 4 reaction wells or tubes: one without any antibiotic or chemical inhibitor against carbapenemases, one containing imipenem without any chemical inhibitor, one containing impenem and tazobactam (which inhibits KPC carbapenemases), one containing imipenem and EDTA (which inhibits VIM, IMP, and NDM carbapenemases). Based on the presence and absence of color change among the 4 reaction wells or tubes, one can differentiate among the 3 classes of carbapenemases. However, differentiation is not possible with this test in isolates that produce more than one class of carbapenemases simultaneously.

Part Description

LP105133-5   Carbapenemase
Carbapenems are famously stable to AmpC β-lactamases and extended-spectrum-β-lactamases. Carbapenemases are a diverse group of β-lactamases that are active not only against the oxyimino-cephalosporins and cephamycins but also against the carbapenems. Carbapenemases were formerly believed to derive only from classes A, B, and D, but a class C carbapenemase has been described. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Carbapenemase

Fully-Specified Name

Component

Carbapenemase

Property

Type

Time

Pt

System

Isolate

Scale

Nom

Method

Carbapenemase Nordmann-Poirel

Additional Names

Long Common Name

Carbapenemase [Type] in Isolate by Carba NP

Short Name

Carbapenemase Islt

Display Name

Carbapenemase Carba NP Nom (Isol)

Consumer Name Alpha Get Info

Carbapenemase

Example Answer List: LL2781-4

Basic Attributes

Class

MICRO

Type

Laboratory

First Released

Version 2.48

Last Updated

Version 2.73 (MIN)

Order vs. Observation

Both

Common Test Rank Get Info

17381

Language Variants Get Info

Related Names

• carbapenem-resistant Klebsiella pneumoniae
• CRE
• CRKP
• Hodge test
• ID
• Infectious Disease
• InfectiousDisease
• Islt
• Isol
• Microbiology
• Nominal
• Point in time
• Random
• Typ

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CodeSystem lookup

https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=74676-8

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