85379-6
Purine/Pyrimidine [Ratio] in Red Blood Cells by Spectrophotometry
Active
Term Description
The purine to pyrimidine ratio is a marker of pyrimidine-5-nucleotidase (P5N) which is an enzyme in the cytoplasm of human red blood cells. The function
of P5N is unknown but a deficiency of the enzyme causes congenital haemolytic anemia. Individuals who are heterozygous for the recessive P5N gene
demonstrate no clinical or hematological abnormalities while those homozygous for the recessive P5N gene have a corresponding 5-30% reduction in
normal enzyme activity. P5N deficiency leads to chronic non-spherocytic haemolytic anaemia associated with mild to moderate hemolysis, increased
basophilic stippling in 5% of the red blood cells and an elevation of red cell glutathione and pyrimidine nucleotides. The diagnosis of P5N deficiency is
based on the change in the level of cytidine and uridine in red blood cells which is normally very low but increases to over 50% in P5N deficient patients.
Measurement of P5N is by light absorption at different wavelengths. In acidic solution pyrimidine nucleotides absorb the maximum amount of light at 280
nanometers (nm). Purine nucleotides absorb the maximum amount of light at 260 nm. The ratio of light absorbed at 260 nm to that at 280 nm in a pool of
red blood cells indicates the relative amount of purines to pyrimidines. The ratio is lower than normal when pyrimidine levels are increased.[PMID:
23992312][ Dacie and Lewis Practical Haematology, 11th edition, Barbara J. Bain, Imelda Bates, Mike A. Laffan, S. Mitchell Lewis, p259]
Source: Regenstrief LOINC
Fully-Specified Name
- Component
- Purine/pyrimidine
- Property
- Ratio
- Time
- Pt
- System
- RBC
- Scale
- Qn
- Method
- Spectrophotometry
Additional Names
- Short Name
- Purine/Pyrimidine RBC Spect-Rto
- Display Name
- Purine/Pyrimidine Spectrophotometry (RBC) [Ratio]
- Consumer Name Alpha Get Info
- Purine/Pyrimidine, Red blood cells
Basic Attributes
- Class
- CHEM
- Type
- Laboratory
- First Released
- Version 2.61
- Last Updated
- Version 2.61
- Order vs. Observation
- Both
Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| es-ES | Spanish (Spain) | Purina/Pirimidina: Synonyms: Cuantitativo |
| es-MX | Spanish (Mexico) | Purina / pirimidina: |
| fr-FR | French (France) | Purine/pyrimidine: |
| it-IT | Italian (Italy) | Purina/Pirimidina: Synonyms: Chimica Globuli rossi Punto nel tempo (episodio) |
| nl-NL | Dutch (Netherlands) | purine/pyrimidine: Synonyms: rode bloedcellen |
| zh-CN | Chinese (China) | 嘌呤/嘧啶: Synonyms: RBC; |
Example Units
| Unit | Source |
|---|---|
| {ratio} | Example UCUM Units |
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