Version 2.80

Descriptions

Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils. NADPH oxidase is the missing enzyme in CGD. From total blood neutrophils can be purified and the NADPH oxidase activity can be measured with different methods in theses cells after activation: Phagocytosis by polymorphonuclear neutrophils and monocytes constitutes an essential arm of host defense against bacterial or fungal infections. The phagocytic process can be separated into several major stages: chemotaxis (migration of phagocytes to inflammatory sites), attachment of particles to the cell surface of phagocytes, ingestion (phagocytosis) and intracellular killing by oxygen-dependent (oxidative burst) and oxygenindependent mechanisms.

Test results are expressed as a normal oxidative index (NOI), which is the ratio of the fluorescence in stimulated cells to the fluorescence expressed in unstimulated cells. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Wikipedia

Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils. The NADPH oxidase enzyme is missing in CGD. From total blood, neutrophils can be purified and the NADPH oxidase activity can be measured with different methods in these cells after activation.

Results are expressed as a normal oxidative index (NOI), which is the ratio of the fluorescence in stimulated cells to the fluorescence expressed in unstimulated cells. The normal range is > 73 NOI. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Neutrophil Oxidative Burst Test

Neutrophils are white blood cells that are critical to the healthy function of the immune system. They are drawn to sites of infection and injury to phagocytose and digest microbes. PMID: 22224774 Neutrophils rely on their ability to generate an oxidative burst to effectively kill microorganisms. The oxidative burst is a series of reactions involving NADPH oxidase that is triggered by receptors when they encounter bacterial peptides such as N-formyl-Met-Leu-Phe (fMLP) and phorbol myristate acetate (PMA) and which results in the production of superoxide anions and reactive oxygen species (ROS). PMID: 22262438 PMID: 10733101 The inability of neutrophils to create an oxidative burst can lead to a decreased ability to fight infection. Chronic granulomatous disease (CGD) is an x-linked or autosomal-recessive disorder where NADPH oxidase is improperly assembled which leads to an inadequate oxidative burst response and increased susceptibility to bacterial and fungal infections. PMID: 22262438 One method of assessing neutrophil function uses flow cytometry to measure the fluorescence of neutrophils that have been stimulated in the presence of the nonfluorescent dye dihydrorhodamine 123 (DHR 123), which, when oxidized, produces the fluorescent compound rhodamine 123. Both the percent of neutrophils having a burst response and the efficacy of that response as reflected by the mean fluorescence intensity (MFI) is measured. Depending on the underlying genetic cause and severity of CGD, some patients have a population of neutrophils that can generate a burst response but with lower MFI compared to healthy controls, while others only have neutrophils that are not capable of generating a response at all. PMID: 26912782 Source: Regenstrief LOINC

Basic Part Properties

Part Name
Neutrophil oxidative burst stimulated/unstimulated
Part Display Name
Neutrophil oxidative burst
Part Type
Component (Describes the core component or analyte measured)
Created On
2012-09-19
Construct for LOINC Short Name
Neutrophil OB

LOINC Terminology Service (API) using HL7® FHIR® Get Info

CodeSystem lookup
https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=LP157356-9

Language Variants Get Info

Tag Language Translation
pl-PL Polish (Poland) Wybuch tlenowy neutrofilów stymulowany/niestymulowany
Synonyms: Wybuch tlenowy neutrofilów
fr-CA French (Canada) Explosion oxydative des neutrophiles stimulé/non stimulés