30903-9
Neutrophil oxidative burst [Presence] in Blood by Flow cytometry (FC)
Active
Part Descriptions
LP29088-9 Neutrophil oxidative burst
White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry.
Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).
Source: ARUP Laboratories, ARUP Laboratories
LP29088-9 Neutrophil oxidative burst
Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils. The NADPH oxidase enzyme is missing in CGD. From total blood, neutrophils can be purified and the NADPH oxidase activity can be measured with different methods in these cells after activation.
Results are expressed as a normal oxidative index (NOI), which is the ratio of the fluorescence in stimulated cells to the fluorescence expressed in unstimulated cells. The normal range is > 73 NOI.
Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details.
Source: Wikipedia, Neutrophil Oxidative Burst Test
LP29088-9 Neutrophil oxidative burst
Neutrophils are white blood cells that are critical to the healthy function of the immune system. They are drawn to sites of infection and injury to phagocytose and digest microbes. PMID: 22224774 Neutrophils rely on their ability to generate an oxidative burst to effectively kill microorganisms. The oxidative burst is a series of reactions involving NADPH oxidase that is triggered by receptors when they encounter bacterial peptides such as N-formyl-Met-Leu-Phe (fMLP) and phorbol myristate acetate (PMA) and which results in the production of superoxide anions and reactive oxygen species (ROS). PMID: 22262438 PMID: 10733101 The inability of neutrophils to create an oxidative burst can lead to a decreased ability to fight infection. Chronic granulomatous disease (CGD) is an x-linked or autosomal-recessive disorder where NADPH oxidase is improperly assembled which leads to an inadequate oxidative burst response and increased susceptibility to bacterial and fungal infections. PMID: 22262438 One method of assessing neutrophil function uses flow cytometry to measure the fluorescence of neutrophils that have been stimulated in the presence of the nonfluorescent dye dihydrorhodamine 123 (DHR 123), which, when oxidized, produces the fluorescent compound rhodamine 123. Both the percent of neutrophils having a burst response and the efficacy of that response as reflected by the mean fluorescence intensity (MFI) is measured. Depending on the underlying genetic cause and severity of CGD, some patients have a population of neutrophils that can generate a burst response but with lower MFI compared to healthy controls, while others only have neutrophils that are not capable of generating a response at all. PMID: 26912782
Source: Regenstrief LOINC
Fully-Specified Name
- Component
- Neutrophil oxidative burst
- Property
- PrThr
- Time
- Pt
- System
- Bld
- Scale
- Ord
- Method
- Flow cytometry
Additional Names
- Short Name
- Neutrophil OB Bld Ql FC
- Display Name
- Neutrophil oxidative burst FC Ql (Bld)
- Consumer Name Alpha Get Info
- Neutrophil oxidative burst, Blood
Basic Attributes
- Class
- HEM/BC
- Type
- Laboratory
- First Released
- Version 2.04
- Last Updated
- Version 2.73
- Change Reason
- The PrThr property is used for LOINC terms whose results are reported using an ordered categorical scale, regardless of whether or not an internal threshold was used to make that determination. This change was approved by the Laboratory LOINC Committee in June 2016.
- Order vs. Observation
- Both
- Common Test Rank Get Info
- 5838
Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| de-AT | German (Austria) | Synonyms: Neutrophil Oxidative Burst |
| es-AR | Spanish (Argentina) | estallido oxidativo de neutrófilos: |
| es-ES | Spanish (Spain) | Estallido oxidativo de neutrófilos: |
| es-MX | Spanish (Mexico) | Explosión oxidativa de neutrófilos: |
| fr-CA | French (Canada) | Explosion oxydative des neutrophiles: |
| fr-FR | French (France) | Explosion oxydative des polynucléaires neutrophiles: |
| fr-BE | French (Belgium) | Explosion oxydative des neutrophiles: |
| it-IT | Italian (Italy) | Neutrofili, esplosione ossidativa: Synonyms: Citometria a flusso (FC) Ematologia (coagulazione) conta differenziale Esplosione ossidativa dei neutrofili Presenza o Soglia Punto nel tempo (episodio) Sangue |
| ko-KR | Korean (Korea, Republic Of) | 호중구 산화 폭발: |
| nl-NL | Dutch (Netherlands) | neutrofiel oxidatieve uitbarsting: |
| pl-PL | Polish (Poland) | Wybuch tlenowy neutrofilów: Synonyms: Neutrofil Wybuch tlenowy neutrofilów |
| pt-BR | Portuguese (Brazil) | Neutrófilo ruptura oxidativa: Synonyms: ; |
| ru-RU | Russian (Russian Federation) | Нейтрофил окислительный взрыв: Synonyms: Кровь Порядковый Точка во времени; |
| tr-TR | Turkish (Turkey) | Nötrofil oksidatif patlama: Synonyms: Akış hücremetresi Mevcut |
| zh-CN | Chinese (China) | 嗜中性粒细胞氧化爆发: Synonyms: PMN; |
LOINC Terminology Service (API) using HL7® FHIR® Get Info
Requests to this service require a free LOINC username and password. Below is a sample of the possible capabilities. See the LOINC Terminology Service documentation for more information.
- CodeSystem lookup
- https:
//fhir.loinc.org/CodeSystem/$lookup?system=http: //loinc.org&code=30903-9
LOINC Copyright
Copyright © 2024 Regenstrief Institute, Inc. All Rights Reserved. To the extent included herein, the LOINC table and LOINC codes are copyright