43292-2

Neutrophil oxidative burst/unstimulated in Blood

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Part Descriptions

LP157356-9   Neutrophil oxidative burst stimulated/unstimulated
Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils. NADPH oxidase is the missing enzyme in CGD. From total blood neutrophils can be purified and the NADPH oxidase activity can be measured with different methods in theses cells after activation: Phagocytosis by polymorphonuclear neutrophils and monocytes constitutes an essential arm of host defense against bacterial or fungal infections. The phagocytic process can be separated into several major stages: chemotaxis (migration of phagocytes to inflammatory sites), attachment of particles to the cell surface of phagocytes, ingestion (phagocytosis) and intracellular killing by oxygen-dependent (oxidative burst) and oxygenindependent mechanisms.

Test results are expressed as a normal oxidative index (NOI), which is the ratio of the fluorescence in stimulated cells to the fluorescence expressed in unstimulated cells. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Wikipedia

LP157356-9   Neutrophil oxidative burst stimulated/unstimulated
Neutrophil Oxidative Burst Test (or Chronic Granulomatous Disease (CGD) Test) is a measure of neutrophil oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overall metabolic integrity of phagocytosing neutrophils. The NADPH oxidase enzyme is missing in CGD. From total blood, neutrophils can be purified and the NADPH oxidase activity can be measured with different methods in these cells after activation.

Results are expressed as a normal oxidative index (NOI), which is the ratio of the fluorescence in stimulated cells to the fluorescence expressed in unstimulated cells. The normal range is > 73 NOI. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Neutrophil Oxidative Burst Test

LP157356-9   Neutrophil oxidative burst stimulated/unstimulated
Neutrophils are white blood cells that are critical to the healthy function of the immune system. They are drawn to sites of infection and injury to phagocytose and digest microbes. PMID: 22224774 Neutrophils rely on their ability to generate an oxidative burst to effectively kill microorganisms. The oxidative burst is a series of reactions involving NADPH oxidase that is triggered by receptors when they encounter bacterial peptides such as N-formyl-Met-Leu-Phe (fMLP) and phorbol myristate acetate (PMA) and which results in the production of superoxide anions and reactive oxygen species (ROS). PMID: 22262438 PMID: 10733101 The inability of neutrophils to create an oxidative burst can lead to a decreased ability to fight infection. Chronic granulomatous disease (CGD) is an x-linked or autosomal-recessive disorder where NADPH oxidase is improperly assembled which leads to an inadequate oxidative burst response and increased susceptibility to bacterial and fungal infections. PMID: 22262438 One method of assessing neutrophil function uses flow cytometry to measure the fluorescence of neutrophils that have been stimulated in the presence of the nonfluorescent dye dihydrorhodamine 123 (DHR 123), which, when oxidized, produces the fluorescent compound rhodamine 123. Both the percent of neutrophils having a burst response and the efficacy of that response as reflected by the mean fluorescence intensity (MFI) is measured. Depending on the underlying genetic cause and severity of CGD, some patients have a population of neutrophils that can generate a burst response but with lower MFI compared to healthy controls, while others only have neutrophils that are not capable of generating a response at all. PMID: 26912782 Source: Regenstrief LOINC

LP157426-0   Neutrophil oxidative burst stimulated
Neutrophils are white blood cells that are critical to the healthy function of the immune system. They are drawn to sites of infection and injury to phagocytose and digest microbes. PMID: 22224774 Neutrophils rely on their ability to generate an oxidative burst to effectively kill microorganisms. The oxidative burst is a series of reactions involving NADPH oxidase that is triggered by receptors when they encounter bacterial peptides such as N-formyl-Met-Leu-Phe (fMLP) and phorbol myristate acetate (PMA) and which results in the production of superoxide anions and reactive oxygen species (ROS). PMID: 22262438 PMID: 10733101 The inability of neutrophils to create an oxidative burst can lead to a decreased ability to fight infection. Chronic granulomatous disease (CGD) is an x-linked or autosomal-recessive disorder where NADPH oxidase is improperly assembled which leads to an inadequate oxidative burst response and increased susceptibility to bacterial and fungal infections. PMID: 22262438 One method of assessing neutrophil function uses flow cytometry to measure the fluorescence of neutrophils that have been stimulated in the presence of the nonfluorescent dye dihydrorhodamine 123 (DHR 123), which, when oxidized, produces the fluorescent compound rhodamine 123. Both the percent of neutrophils having a burst response and the efficacy of that response as reflected by the mean fluorescence intensity (MFI) is measured. Depending on the underlying genetic cause and severity of CGD, some patients have a population of neutrophils that can generate a burst response but with lower MFI compared to healthy controls, while others only have neutrophils that are not capable of generating a response at all. PMID: 26912782 Source: Regenstrief LOINC

Fully-Specified Name

Component

Neutrophil oxidative burst stimulated/unstimulated

Property

RelACnc

Time

Pt

System

Bld

Scale

Qn

Method

Additional Names

Long Common Name

Neutrophil oxidative burst/unstimulated in Blood

Short Name

Neutrophil OB Bld

Display Name

Neutrophil oxidative burst stim/unstimulated (Bld) [Rel units/Vol]

Consumer Name Alpha Get Info

Neutrophil oxidative burst stim/unstimulated, Blood

Basic Attributes

Class

HEM/BC

Type

Laboratory

First Released

Version 2.17

Last Updated

Version 2.73 (MIN)

Order vs. Observation

Both

Common Test Rank Get Info

12847

Language Variants Get Info

Related Names

• Blood
• CGD test
• Chronic granulomatous disease test
• HEMATOLOGY/CELL COUNTS
• Neut
• Neutr
• Neutrophil OB
• Neutrophils
• PMN
• PNM
• Point in time
• QNT
• Quan
• Quant
• Quantitative
• Random
• RlACnc
• WB
• Whole blood

Example Units

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Copyright © 2025 Regenstrief Institute, Inc. All Rights Reserved. To the extent included herein, the LOINC table and LOINC codes are copyright © Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. See https://loinc.org/license for the full LOINC copyright and license.