LOINC
Version 2.67

81725-4Glutamate decarboxylase 65 Ab [Presence] in Serum by Line blotActive

Term Description

This term was created for, but is not limited in use to, the submitter's test strip, a membrane strip pre-coated with a specific set of antigens in parallel lines. If antibodies to any of those antigens are present in the patient's serum, they will bind to the specific antigen. The bound patient antibodies are then detected using labelled anti-human antibodies. Various manufacturers classify this method as an immunoblot technique. LOINC uses the immunoblot (IB) method to mean that some electrophoretic/chromatographic separation occurs such that position (size) of the band is part of the assessment (as in Western blot). Because this assay does not involve a separation step but rather uses strips with pre-fixed antigens, the LOINC Method is Line blot.
Source: Regenstrief LOINC

Part Description

LP18489-2   Glutamate decarboxylase 65
Glutamate decarboxylase 65 (GAD65) antibody is useful to diagnose insulin dependent diabetes mellitus (IDDM, Type I diabetes), to assess the risk for developing IDDM, to predict the onset of IDDM and the risk of developing related endocrine disorders such as thyroiditis. Before clinical onset, Type I diabetes is characterized by lymphocytic infiltration of the islet cells and by circulating autoantibodies against a variety of islet cell antigens including GAD-65, IA-2 (a tyrosine phosphatase-like protein), and insulin (IAA). GAD has two isoforms, GAD65 and GAD67, which are highly homologous but found in distinct subsets of patients. [PMID: 7532143] GAD65 antibodies are specific to patients with insulin-dependent diabetes. The presence of GAD antibodies in a patient recently diagnosed with diabetes indicates a high risk of requiring insulin within three years. [PMID: 11114103] GAD67 is important to the maturation of perisomatic innervation.( http://www.sciencedirect.com/science/article/pii/S0896627307003728) GAD65 are also associated with paraneoplastic neurologic syndrome (PNS) which is an autoimmune disorder that is manifested by progressive neurological syndromes of any combination of the central, peripheral and autonomic nervous systems. In addition, the different PNS antibodies are associated with certain cancers and can assist in identifying where an unknown tumor is located. [PMID: 22412264] Glutamic acid decarboxylase 65 antibody are associated with stiff person syndrome, cerebellar ataxia encephalitis and other neurological diseases. [PMID: 22405456] Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Regenstrief LOINC, Glutamate (Wikipedia)

Fully-Specified Name

Component
Glutamate decarboxylase 65 Ab
Property
PrThr
Time
Pt
System
Ser
Scale
Ord
Method
Line blot

Additional Names

Short Name
GAD65 Ab Ser Ql Line blot
Display Name
Glutamate decarboxylase 65 Ab Line blot Ql (S)

Basic Attributes

Class
SERO
Type
Laboratory
First Released
Version 2.56
Last Updated
Version 2.64
Change Reason
This term was created for an assay that uses a membrane strip pre-coated with a specific set of antigens in parallel lines. Because the antigens are seperated by electrophoresis/chromatographic separation during manufacturing, not during the test procedure itself, this test was assigned a LOINC Method of Immunoassay (IA) and not Immunoblot (IB). However, upon further review of IA, IB, and Immunoblot strip methodologies and regulatory distinctions between these, we changed the Method from IA to Line blot so that users can distinguish between these three Methods. The meaning of the term has not changed since it was created for an IB strip assay as described in the term description.
Order vs. Observation
Both

Example Answer List LL360-9

Source: Regenstrief Institute

Answer Code Score Answer ID
Positive Copyright http://snomed.info/sct ID:10828004 Positive (qualifier value) LA6576-8
Negative Copyright http://snomed.info/sct ID:260385009 Negative (qualifier value) LA6577-6

Member of these Groups

LG27801-6 Glutamate decarboxylase 65 Ab|PrThr|Pt|ANYBldSerPl

Language Variants Get Info

zh-CNChinese (CHINA)
谷氨酸脱羧酶-65 抗体:存在情况或阈值:时间点:血清:序数型:线点印迹法
nl-NLDutch (NETHERLANDS)
glutamaatdecarboxylase 65 As:aanwezigheid:moment:serum:ordinaal:lijnblot
fr-CAFrench (CANADA)
Glutamate décarboxylase 65 , Ac:Présence-Seuil:Temps ponctuel:Sérum:Ordinal:Immunoessai sur bandelette
fr-FRFrench (FRANCE)
Glutamate décarboxylase 65 anticorps:Présence/Seuil:Ponctuel:Sérum:Qualitatif:Blot en ligne
es-ESSpanish (SPAIN)
Glutamato decarboxilasa 65 Anticuerpos:PrThr:Punto temporal:Suero:Ord:Line blot

LOINC FHIR® API Example - CodeSystem Request Get Info

https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=81725-4