LP30813-7
Eosin-5-Maleimide binding
Active
Description
Eosin-5-maleimide (EMA) is a fluorescent dye used in flow cytometry to screen for hereditary spherocytosis (HS). Patients with HS have red blood cells (RBC) that are overly rigid and misshapen and are removed by the spleen for destruction which leads to anemia, jaundice and splenomegaly. EMA binds covalently to Lys-430 on the first extracellular loop of the Band 3 protein on RBCs. The cytoplasmic portion of Band 3 communicates with ankyrin and protein 4.2 that are crosslinked with the spectrin-based cytoskeleton that acts to stabilize the cell membrane. When band 3 is deficient or abnormal on RBCs fluorescence using EMA may be reduced. The mean fluorescent intensity (MFI) of the patient and control cells is measured and the result is expressed as a ratio of patient MFI to control MFI.[Dacie and Lewis Practical Haematology, 11th edition, Barbara J. Bain, Imelda Bates, Mike A. Laffan, S. Mitchell Lewis, pp250-251]MedlinePlus Condition: hereditary-spherocytosis] Source: Regenstrief LOINC
Basic Part Properties
- Part Name
- Eosin-5-Maleimide binding
- Part Display Name
- Eosin-5-Maleimide binding
- Part Type
- Component (Describes the core component or analyte measured)
- Created On
- 2002-11-07
- Construct for LOINC Short Name
- EMA Bind
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Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| zh-CN | Chinese (China) | 伊红-5-马来酰亚胺结合 Synonyms: EMA; |
| es-ES | Spanish (Spain) | fijación de eosina-5-maleimida |
| it-IT | Italian (Italy) | Eosina-5-Maleimide legante |
| tr-TR | Turkish (Turkey) | Eosin-5-Maleimid bağlayıcı |
| ru-RU | Russian (Russian Federation) | Эозин-5-малеимид связывание |
| fr-BE | French (Belgium) | Liaison à l'éosine-5-maléimide |
| fr-CA | French (Canada) | Liaison à l'éosine-5-maléimide |
| nl-NL | Dutch (Netherlands) | eosine-5-maleïmide binding |
| el-GR | Greek (Greece) | Δέσμευση ηωσίν-5-μαλεϊμιδικού Synonyms: Δέσμευση ηωσίν-5-μαλεϊμιδικού |
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