LP32729-3
Hoechst stain
Active
Description
The Hoechst stains are part of a family of fluorescent stains for labelling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis -benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit bluecyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. Because the Hoechst stains bind to DNA, they can disrupt DNA replication during cell division. Consequently they are potentially mutagenic and carcinogenic. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia, Hoechst stain (Wikipedia)
Basic Part Properties
- Part Name
- Hoechst stain
- Part Display Name
- Hoechst stain
- Part Type
- Method (Describes the method by which the test was performed)
- Created On
- 2003-10-28
- Construct for LOINC Short Name
- Hoechst Stn
LOINC Terminology Service (API) using HL7® FHIR® Get Info
Requests to this service require a free LOINC username and password. Below is a sample of the possible capabilities. See the LOINC Terminology Service documentation for more information.
Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| zh-CN | Chinese (China) | Hoechst 染色法 Synonyms: Hoechst DNA 荧光染色法; |
| fr-CA | French (Canada) | Coloration Hoechst |
| es-ES | Spanish (Spain) | Manchas de Hoechst |
| it-IT | Italian (Italy) | Hoechst, colorazione Synonyms: Colorazioni Hoechst |
| tr-TR | Turkish (Turkey) | Hoechst boyama |
| nl-NL | Dutch (Netherlands) | Hoechst-kleuring |
| fr-BE | French (Belgium) | Coloration Hoechst |
| fr-FR | French (France) | Coloration Hoechst |
| pl-PL | Polish (Poland) | barwienie Hoechst'a |
| el-GR | Greek (Greece) | Χρώση Hoechst Synonyms: Χρώση Hoechst |
LOINC Copyright
Copyright © Regenstrief Institute, Inc. All Rights Reserved. To the extent included herein, the LOINC table and LOINC codes are copyright © Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. See https://