LP6443-8
Periodic acid-Schiff stain
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Description
Periodic acid-Schiff (PAS) is a staining method used in histology and pathology. This method is primarily used to identify glycogen in tissues. The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS staining is mainly used for staining structures containing high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in eg. connective tissues, mucus, and basal laminae. PAS staining can be used to distinguish between different types of glycogen storage diseases. J. F. A. McManus PAS Method For Glycogen Fixation: 10% Buffered Neutral Formalin Section: Paraffin, @ 6 micrometres Staining Procedure: Digest using a diastase, hyaluronidase, or sialidase procedure 1. Deparaffinize and hydrate to distilled water 2. Oxidize in Periodic Acid, 0.5%, 5 minutes and rinse in distilled water 3. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia , Periodic acid-Schiff stain (Wikipedia)
Basic Part Properties
- Part Name
- Periodic acid-Schiff stain
- Part Display Name
- Periodic acid-Schiff stain
- Part Type
- Method (Describes the method by which the test was performed)
- Created On
- 2000-05-03
- Construct for LOINC Short Name
- PAS Stn
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- CodeSystem lookup
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Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| zh-CN | Chinese (China) | PAS 染色法 Synonyms: 过碘酸雪夫氏反应染色法 |
| es-ES | Spanish (Spain) | Tinción ácido peryódico de Schiff |
| it-IT | Italian (Italy) | Acido periodico-Schiff, colorazione Synonyms: Colorazione acido periodico-Schiff |
| et-EE | Estonian (Estonia) | PAS värv |
| tr-TR | Turkish (Turkey) | Periyodik asit-Schiff boyama |
| fr-BE | French (Belgium) | Coloration à l'acide périodique-Schiff Synonyms: Coloration. Périodique-Schiff |
| fr-CA | French (Canada) | Coloration à l'acide périodique-Schiff |
| nl-NL | Dutch (Netherlands) | perjoodzuur-Schiff-kleuring |
| pl-PL | Polish (Poland) | barwienie PAS |
| el-GR | Greek (Greece) | Χρώση Schiff περιοδικού οξέος Synonyms: Χρώση Schiff περιοδικού οξέος |
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