74311-2

PRRSV ORF5 gene sequence [Nucleotide sequence] in Isolate by Sequencing

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Term Description

Sequencing of ORF5 gene in PRRSV and reporting the actual base sequence. The submitter's lab performs reverse transcriptase PCR on various specimens, including tissue, serum, oral fluid, and semen, to detect and isolate the ORF5 gene. PCR products are then used for sequencing.

Part Descriptions

LP150045-5 Sequencing
Sequencing is a method used to determine the sequence of individual genes, larger genetic regions (i.e. clusters of genes or operons), full chromosomes or entire genomes. Historically, most sequencing has been performed using the chain termination method developed by Frederick Sanger in 1977. PMID: 271968 Sequencing technologies have improved dramatically, making them cheaper, faster, and more accurate. Next-generation sequencing (NGS), also known as high-throughput sequencing, deep sequencing, and second-generation sequencing, is a type of technology that uses parallel sequencing of multiple small fragments of DNA to determine sequence. This "high-throughput" technology has increased the speed and amount of DNA sequenced at a significantly reduced cost. PMID: 18576944 Several NGS platforms (ie, sequencing instruments and associated reagents) have been developed. Third-generation sequencing is another methodology currently under development that uses parallel sequencing similar to NGS. In contrast to NGS, third-generation sequencing uses single DNA molecules rather than amplified DNA as a template. PMID: 20858600 Source: Regenstrief LOINC

LP175629-7 Porcine reproductive and respiratory syndrome virus ORF5 gene sequence
Porcine reproductive and respiratory syndrome virus (PRRSV), also referred to as Blue-Ear Pig disease, causes significant disease and can lead to reproductive failures such as weak fetuses, mummified fetuses, abortions or still borns. PRRSV is an RNA virus and belongs to the order Nidovirales, family Arteriviridae and the genus Arterivirus. PRRSV is spread among all ages of pigs and herds through direct and indirect contact. PRRSV is considered one of the most economically important infectious agents in the swine industry worldwide. The continual changing genomic characteristics of the various strains of this virus make testing of herds an important part of disease management. PMID: 26646630 Source: Regenstrief LOINC

LOINC Names Get Info

Fully-Specified Name: Porcine reproductive and respiratory syndrome virus ORF5 gene sequence:Seq:Pt:Isolate:Nom:Sequencing
Long Common Name: PRRSV ORF5 gene sequence [Nucleotide sequence] in Isolate by Sequencing
Short Name: PRRSV ORF5 seq Islt-Seq
Display Name: PRRSV ORF5 gene sequence sequence Nom (Isol)
Consumer Name Alpha Get Info: PRRSV ORF5 gene Sequence

Part Model Get Info

Component
Porcine reproductive and respiratory syndrome virus ORF5 gene sequence
LP175629-7
- Analyte
  Porcine reproductive and respiratory syndrome virus ORF5 gene sequence
  LP175629-7
  - Component Numerator
    Porcine reproductive and respiratory syndrome virus ORF5 gene sequence
    LP175629-7
    - Component Numerator Core
      Porcine reproductive and respiratory syndrome virus ORF5 gene sequence
      LP175629-7
    - Component Numerator Core Suffix
      NULL
  - Component Denominator
    NULL
    - Component Denominator Core
      NULL
    - Component Denominator Core Suffix
      NULL
- Challenge
  NULL
- Adjustment
  NULL
- Count
  NULL
Property
Seq
LP62164-6
Time
Pt
LP6960-1
- Time Core
  Pt
  LP6960-1
- Time Modifier
  NULL
System
Isolate
LP7370-2
- System Core
  Isolate
  LP7370-2
- Super System
  NULL
Scale
Nom
LP7750-5
Method
Sequencing
LP150045-5

Basic Attributes

Class: MICRO
Type: Laboratory
First Released: Version 2.46
Last Updated: Version 2.64 (MIN)
Change Reason: Abbreviated "Porcine reproductive and respiratory syndrome virus" to "PRRSV" in the Long Common Name. PRRSV is shorter and more commonly used in test names.
Order vs. Observation: Both

Language Variants Get Info

Tag	Language	Translation
cs-CZ	Czech (Czechia)	PRRSV ORF5 genová sekvence:Nukleotidová sekvence:Časový bod:Izolát:Nominální:Sekvenace
el-GR	Greek (Greece)	Αλληλουχία γονιδίου ORF5 του ιού του αναπαραγωγικού και αναπνευστικού συνδρόμου των χοίρων:Seq:Pt:Απομονωμένο στέλεχος:Nom:Αλληλούχιση Synonyms: MICRO Nom Pt Seq Αλληλουχία γονιδίου ORF5 του ιού του αναπαραγωγικού και αναπνευστικού συνδρόμου των χοίρων Αλληλούχιση Απομονωμένο στέλεχος Γονίδιο Γονίδιο ORF5 του ιού του αναπαραγωγικού και αναπνευστικού συνδρόμου των χοίρων Ιός Ιός του αναπαραγωγικού και αναπνευστικού συνδρόμου των χοίρων Των χοίρων
es-ES	Spanish (Spain)	Secuencia del gen ORF5 del virus del síndrome reproductivo y respiratorio porcino:Secuencia de nucleótidos:Punto temporal:Aislado:Nom:Secuenciación
es-MX	Spanish (Mexico)	Secuencia del gen ORF5 del virus del síndrome respiratorio y reproductivo porcino:Secuencia de nucleótidos:Punto temporal:Aislado:Nominal:Secuenciación
it-IT	Italian (Italy)	Virus della sindrome riproduttiva e respiratoria del suino, sequenziamento del gene ORF5:Seq:Pt:Isolato:Nom:Sequenziamento Synonyms: Gene ORF5 virus PRRS Microbiologia Punto nel tempo (episodio) Sequenza nucleotidica Sequenziamento del gene ORF5 virus PRRS Virus PRRS
nl-NL	Dutch (Netherlands)	'porcine' reproductief en respiratoir syndroomvirus ORF5-gensequentie:nucleotidevolgorde:moment:isolaat:nominaal:sequencing Synonyms: porcine voortplantings- en respiratoir syndroom virus van varken; PRRSV
pl-PL	Polish (Poland)	Sekwencja genu ORF5 wirusa zespołu rozrodczo-oddechowego świń:sekwencja nukleotydów:punkt w czasie:izolat:skala nominalna:sekwencjonowanie Synonyms: Gen ORF5 PRRSV Sekwencja genu ORF5 PRRSV wynik kategorialny
tr-TR	Turkish (Turkey)	Porsin reprodüktif ve respiratuvar sendrom virüs ORF5 gen dizisi:Sek:Zmlı:İzolat:Snf:Sekanslama Synonyms: Dizi tayini Sekans
zh-CN	Chinese (China)	猪生殖与呼吸综合征病毒 ORF5 基因序列:序列:时间点:分离株:名义型:序列测定 Synonyms: Lelystad 病毒;PRRS;SIRSV;兰耳病;新猪病;母猪后期流产;猪不育和呼吸综合症;猪不育和呼吸综合症（Swine infertility and respiratory syndrome，SIRSV）;猪生殖与呼吸系统综合症;猪生殖与呼吸综合症（Porcine reproductive and respiratory syndrome，PRRS）;猪繁殖与呼吸综合征;猪蓝耳病;猪蓝色病;神秘仔猪病;神秘猪病;莱利斯塔猪病毒（Lelystad Virus，LV）;蓝耳猪病;蓝耳病;蓝色流产症 PRRSV;猪生殖呼吸综合征病毒;猪呼吸繁殖综合征病毒;猪繁殖与呼吸道综合征病毒;猪繁殖和呼吸障碍综合征;Porcine Reproductive and Respiratory Syndrome;PRRS;蓝耳病;Blue Ear Disease;可译框架;可读框;开放阅读框;开放读码框;开放读码框架;编码框;ORF;open reading frame 传染毒;传染毒素;滤过性病原体;病毒类;过滤性病原体分离物;分离菌;分离菌株;隔离种群分类型应答;分类型结果;名义性;名称型;名词型;名词性;标称性;没有自然次序的名义型或分类型应答序列分析;测序微生物学;微生物学试验;微生物学试验（培养、DNA、抗原及抗体）时刻;随机;随意;瞬间核苷酸序列猪的猪科猪科的遗传基因;遗传因子;吉恩;生物基因

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