74861-6

Chromosome 17 copy number/nucleus in Tissue by FISH

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Term Description

The average number of Chromosome 17 fluorescent signals in nuclei are determined in order to calculate the average ratio of HER2 to Chromosome 17 copy number LOINC 49683-6. This term is based on, but not limited in use to, the PathVysion HER-2 DNA Probe Kit, which detects amplification of the ERBB2 gene (HER2 locus) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded human breast and gastric cancer tissue specimens. The kit contains two probes: one specific to the HER-2/neu gene locus (ERBB2 gene, 17q11.2-q12) and another targeting the alpha satellite DNA sequence at the centromeric region of chromosome 17 (17p11.1-q11.1), called CEP17 (Chromosome Enumeration Probe 17). The average number of HER2 signals LOINC 74860-8 along with CEP17 signals are determined by microscopic examination within the same set of nuclei. The specimen is positive for ERBB2 gene applification if the mean ratio of HER2 to CEP17 signals per nucleus is greater than or equal to 2.00.

Part Descriptions

LP182348-5 Chromosome 17 copy number
The Chromosome 17 DNA probe marks the centromeric region of chromosome 17 and is used as an internal control and to account for aneusomy (varying number) of chromosome 17. Source: Regenstrief LOINC

LP62864-1 FISH
FISH (fluorescence in situ hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes. FISH is often used for finding specific features in DNA for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues. Copyright Text is available under the Creative Commons Attribution/Share-Alike License. See http://creativecommons.org/licenses/by-sa/3.0/ for details. Source: Wikipedia, FISH

LOINC Names Get Info

Fully-Specified Name: Chromosome 17 copy number/nucleus:EntNum:Pt:Tiss:Qn:FISH
Long Common Name: Chromosome 17 copy number/nucleus in Tissue by FISH
Short Name: Chr 17 copy num/nucleus EntNum Tiss FISH
Display Name: Chromosome 17 copy number/nucleus FISH (Tiss) [Entitic number]
Consumer Name Alpha Get Info: Chromosome 17 copy number/nucleus, Tissue

Part Model Get Info

Component
Chromosome 17 copy number/nucleus
LP285721-9
- Analyte
  Chromosome 17 copy number/nucleus
  LP285721-9
  - Component Numerator
    Chromosome 17 copy number
    LP182348-5
    - Component Numerator Core
      Chromosome 17 copy number
      LP182348-5
    - Component Numerator Core Suffix
      NULL
  - Component Denominator
    nucleus
    LP182349-3
    - Component Denominator Core
      nucleus
      LP182349-3
    - Component Denominator Core Suffix
      NULL
- Challenge
  NULL
- Adjustment
  NULL
- Count
  NULL
Property
EntNum
LP6806-6
Time
Pt
LP6960-1
- Time Core
  Pt
  LP6960-1
- Time Modifier
  NULL
System
Tiss
LP7641-6
- System Core
  Tiss
  LP7641-6
- Super System
  NULL
Scale
Qn
LP7753-9
Method
FISH
LP62864-1

Basic Attributes

Class: MOLPATH
Type: Laboratory
First Released: Version 2.48
Last Updated: Version 2.73 (MIN)
Change Reason: Updated Component from "CEP17 signals" to "Chromosome 17 copy number" to align with current nomenclature and similar terms
Order vs. Observation: Observation
Common Test Rank Get Info: 11691

Member of these Panels

LOINC	Long Common Name
85904-1	Cancer pathology panel - Breast cancer specimen by CAP cancer protocols
74885-5	ERBB2 gene (HER2) duplication associated observations panel - Tissue by FISH

Language Variants Get Info

Tag	Language	Translation
cs-CZ	Czech (Czechia)	Chromozom 17 počet kopií/jádro:Entitní počet:Časový bod:Tkáně a stěry:Kvantitativní:Fluorescenční in situ hybridizace (FISH)
el-GR	Greek (Greece)	Αριθμός αντιγράφων χρωμοσώματος 17/πυρήνας:EntNum:Pt:Ιστός:Qn:Φθορίζουσα υβριδοποίηση in situ Synonyms: EntNum MOLPATH Pt Qn Αριθμός Αριθμός αντιγράφων χρωμοσώματος 17 Ιστός πυρήνας Φθορίζουσα υβριδοποίηση in situ Χρωμόσωμα
es-ES	Spanish (Spain)	Señales de CEP17/núcleo:Número entítico:Punto temporal:Tejido:Qn:Hibridación in situ fluoresente (FISH) Synonyms: Cuantitativo
es-MX	Spanish (Mexico)	Número de copias / núcleo del cromosoma 17:Número entitic:Punto temporal:Tejido y frotis:Cuantitativo:Hibridación fluorescente in situ (FISH)
fr-FR	French (France)	Chromosome 17 nombre de copie/noyaux:Nombre/entité:Ponctuel:Tissu:Numérique:FISH
it-IT	Italian (Italy)	CEP17, segnali/nucleo:EntNum:Pt:Tessuto:Qn:FISH Synonyms: Ibridazione in situ fluorescente (FISH) Numero entitico Patologia molecolare Punto nel tempo (episodio) Tessuto & Strisci
nl-NL	Dutch (Netherlands)	aantal centromeren van chromosoom 17/kern:aantal/eenheid:moment:weefsel en uitstrijkjes:kwantitatief:FISH Synonyms: CEP17 nucleus
pl-PL	Polish (Poland)	Liczba kopii chromosomu 17/jądro:liczba zawarta w:punkt w czasie:tkanka i rozmazy:ilościowy:FISH Synonyms: fluorescencyjna hybrydyzacja in situ
tr-TR	Turkish (Turkey)	CEP17 sinyalleri/çekirdek:EntSay:Zmlı:Doku:Kant:FISH
zh-CN	Chinese (China)	染色体 17 拷贝数/核:实体数量:时间点:组织与涂片:定量型:FISH Synonyms: Fluorescent in situ hybridization;荧光原位杂交个数;数目分子病理学;分子病理学试验可用数量表示的;定量性;数值型;数量型;连续数值型标尺实体计数时刻;随机;随意;瞬间未作说明的组织;组织;组织 & 涂片染色体 17 （17号染色体）拷贝（副本）数（数量、计数）;17号染色体拷贝（副本）数（数量、计数）;CEP17;CEP17 信号染色体二体型+染色体三体型细胞核

Example Units

Unit	Source
{#}	Example UCUM Units

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LOINC Copyright

Copyright © Regenstrief Institute, Inc. All Rights Reserved. To the extent included herein, the LOINC table and LOINC codes are copyright © Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. See https://loinc.org/license for the full LOINC copyright and license.