74861-6
Chromosome 17 copy number/nucleus in Tissue by FISH
Active
Term Description
The average number of Chromosome 17 fluorescent signals in nuclei are determined in order to calculate the average ratio of HER2 to Chromosome 17 copy number [LOINC: 49683-6]. This term is based on, but not limited in use to, the PathVysion HER-2 DNA Probe Kit, which detects amplification of the ERBB2 gene (HER2 locus) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded human breast and gastric cancer tissue specimens. The kit contains two probes: one specific to the HER-2/neu gene locus (ERBB2 gene, 17q11.2-q12) and another targeting the alpha satellite DNA sequence at the centromeric region of chromosome 17 (17p11.1-q11.1), called CEP17 (Chromosome Enumeration Probe 17). The average number of HER2 signals [LOINC: 74860-8] along with CEP17 signals are determined by microscopic examination within the same set of nuclei. The specimen is positive for ERBB2 gene applification if the mean ratio of HER2 to CEP17 signals per nucleus is greater than or equal to 2.00.
Source: Regenstrief LOINC
Part Descriptions
LP182348-5 Chromosome 17 copy number
The Chromosome 17 DNA probe marks the centromeric region of chromosome 17 and is used as an internal control and to account for aneusomy (varying number) of chromosome 17.
Source: Regenstrief LOINC
LP62864-1 FISH
FISH (fluorescence in situ hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes. FISH is often used for finding specific features in DNA for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.
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Source: Wikipedia, FISH
Fully-Specified Name
- Component
- Chromosome 17 copy number/nucleus
- Property
- EntNum
- Time
- Pt
- System
- Tiss
- Scale
- Qn
- Method
- FISH
Additional Names
- Short Name
- Chr 17 copy num/nucleus EntNum Tiss FISH
- Display Name
- Chromosome 17 copy number/nucleus FISH (Tiss) [Entitic number]
- Consumer Name Alpha Get Info
- Chromosome 17 copy number/nucleus, Tissue
Basic Attributes
- Class
- MOLPATH
- Type
- Laboratory
- First Released
- Version 2.48
- Last Updated
- Version 2.73
- Change Reason
- Updated Component from "CEP17 signals" to "Chromosome 17 copy number" to align with current nomenclature and similar terms
- Order vs. Observation
- Observation
- Common Test Rank Get Info
- 11691
Member of these Panels
| LOINC | Long Common Name |
|---|---|
| 85904-1 | Cancer pathology panel - Breast cancer specimen by CAP cancer protocols |
| 74885-5 | ERBB2 gene (HER2) duplication associated observations panel - Tissue by FISH |
Language Variants Get Info
| Tag | Language | Translation |
|---|---|---|
| es-ES | Spanish (Spain) | Señales de CEP17/núcleo: Synonyms: Cuantitativo |
| es-MX | Spanish (Mexico) | Número de copias / núcleo del cromosoma 17: |
| fr-FR | French (France) | Chromosome 17 nombre de copie/noyaux: |
| it-IT | Italian (Italy) | CEP17, segnali/nucleo: Synonyms: Ibridazione in situ fluorescente (FISH) Numero entitico Patologia molecolare Punto nel tempo (episodio) Tessuto & |
| nl-NL | Dutch (Netherlands) | aantal centromeren van chromosoom 17/kern: Synonyms: CEP17 nucleus |
| pl-PL | Polish (Poland) | Liczba kopii chromosomu 17/jądro: |
| pt-BR | Portuguese (Brazil) | CEP17 sinais/núcleo: |
| tr-TR | Turkish (Turkey) | CEP17 sinyalleri/çekirdek: |
| zh-CN | Chinese (China) | 染色体 17 拷贝数/核: Synonyms: Fluorescent in situ hybridization; |
Example Units
| Unit | Source |
|---|---|
| {#} | Example UCUM Units |
LOINC Terminology Service (API) using HL7® FHIR® Get Info
Requests to this service require a free LOINC username and password. Below is a sample of the possible capabilities. See the LOINC Terminology Service documentation for more information.
- CodeSystem lookup
- https:
//fhir.loinc.org/CodeSystem/$lookup?system=http: //loinc.org&code=74861-6
LOINC Copyright
Copyright © 2024 Regenstrief Institute, Inc. All Rights Reserved. To the extent included herein, the LOINC table and LOINC codes are copyright